Biofunctional Magnetic Nanoparticles for Pathogen Detection
Patent Information:
Filing Country: USA
Application No: 11/159882
Filing Date: 23 June 2005
Patent No: 7754444
Issue Date: 13 July 2010
Inventor 1: Mr. Hongwei, GU
Inventor 2: Dr. Pak Leung, HO
Inventor 3: Prof. Bing, XU

Existing methods for detection of pathogens at ultra-low concentration are usually time-consuming. This is mainly due to the lack of general and satisfactory assay that could detect bacteria at concentrations < 102 cfu/ml without pre-enriching bacteria via a culture process. A protocol is now invented, making detection of Gram-positive and Gram-negative bacteria within 1 hour at concentration of around 10 cfu/ml. This new protocol uses biofunctional magnetic nanoparticles for capture and detection of Gram-positive and Gram-negative bacteria. Data indicated that this new method performs at an order of magnitude more sensitive than the current best assay for bacteria detection based on luminescence, which the detection limit is 180 cfu/ml. By combining magnetic nanoparticles with a specific ligand-receptor interaction, a sensitive and quick assay is introduced to allow bacteria to be captured and analyzed. It offers benefits in clinical diagnosis, food quality monitoring and bio-terrorism prevention. The high sensitivity provided by the biofunctional magnetic nanoparticles will also allow detecting other biological substrates at exceedingly low concentration.
Potential Application   Advantage
- Clinical diagnosis,
- Food quality monitoring
- Bio-terrorism prevention
- Environmental monitoring
  1. Instant
2. Sensitive
3. Low production cost
4. Simple to use
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