Method and System for Real Time Quantification and Monitoring of Nucleic Acid Amplification Using Electroconductive or Electrochemically Active Labels
Patent Information:
1. Filing Country: USA
Application No: 12/377124
Filing Date: 11 February 2009
Patent No: 8465926
Issue Date: 18 June 2013
2. Filing Country: USA
Application No: 13/169055
Filing Date: 27 June 2011
Patent No: 8975025
Issue Date: 10 March 2015
Inventor 1: Prof. I Ming, HSING
Inventor 2: Dr. Thomas Ming Hung, LEE
Inventor 3: Mr. Stephen Siu Wai, YEUNG

The present invention describes an electrochemistry-based real-time polymerase chain reaction (PCR). This invention aims to address the deficiencies inherent in the prior art of optical-based (e.g. fluorescence) real-time PCR method by utilizing the novel strategies based on redox-labeled peptide nucleic acid probe and solid phase extension of oligonucletotide capture probe with redox markers to realize the real time electrochemical PCR technique. This invention also contains a microchip platform strategy to carry out the electrochemistry-based real-time PCR on a portable chip based system. This electrochemical approach utilizes simple and miniaturizable instrumentation compared to the bulky and sophisticated optics required in the fluorescence-based schemes. This invention can be used for the real-time bioanalyte (e.g. water pathogens, E. Coli) detection in a decentralized environment.
Potential Application   Advantage
- Market of this new technology will cover scientific research, clinical diagnostics, environmental monitoring, food testing and biodefense applications.
- Two types of electrochemistry-based real time PCR instruments are possible for different applications:-
(a) Bench-top type - for hospitals, research/testing/teaching laboratories, government lab for food/drug testing which require real time PCR facilities.
(b) Portable real-time PCR analyzer - promises nucleic acids biosensor for decentralized and point-of-care testing, including real-time bioanalyte detection such as:
- genetic diseases analysis;
- viral quantification;
- pathogen detection / identification;
- environmental monitoring;
- drug monitoring
  1. Simple instrumentation and cost effective
2. Electroactive reporters cost less than the fluorescence-based counterparts
3. Procedure for multiplex DNA analysis in a microarray format is simple
4. Real-time - allows simultaneous amplification and multiplexing detection in short assay time
5. High throughput
6. Accurate - uses threshold cycle detection in order to have accurate quantification
7. Portable - allows decentralizing analysis from central laboratory to on-spot detection
8. Wide dynamic range of quantification

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HKUST R and D Corporation Ltd
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