Fluorescent Water-Soluable Conjugated Polyene Compounds that Exhibit Aggregation Induced Emission and Methods of Making and Using Same
Patent Information:
Filing Country: USA
Application No: 12/453892
Filing Date: 26 May 2009
Patent No: 8129111
Issue Date: 06 March 2012
Inventor 1: Prof. Benzhong, TANG
Inventor 2: Dr. Yuning, HONG

Biosensing processes such as molecular beacons require much effort to covalently label or mark biomolecules. The tedious process for traditional protein and DNA assays and the self-quenching problem of conventional dyes suggest the need for the highly sophisticated sensing materials. This invention presents a label-free DNA assay system using a simple dye with aggregation-induced emission (AIE) characteristics as the fluorescent bioprobe. All the polyenes that we provide show aggregation-induced emission but not quenching due to their special structures. Besides, all the assays do not require pre-modification of the biomacromolecules. The distinct AIE feature of TTAPE in particular enables real-time monitoring of folding process of G1 in the absence of any pre-attached fluorogenic labels on the DNA strand. When TTAPE is bound to a guanine-rich DNA strand (G1) via electrostatic attraction, its intramolecular rotation is restricted and its emission is turned on. TTAPE works as a sensitive poststaining agent for PAGE visualization of G1. The dye is highly affinitive to a secondary structure of G1 called G-quadruplex. The bathochromic shift involved in the G1 folding process allows spectral discrimination of the G-quadruplex from other DNA structures. Specificity to K+-induced and stabilized quadruplex structure allows TTAPE to be used as a K+ biosensor. A counterpart of TTAPE, SATPE, on the other hand shows higher affinity to human serum albumin (HSA), the main protein in human urine. Such feature allows SATPE to serve as a probe for protein detection in aqueous media. SATPE may therefore be used for urinary protein detection, helping to detection proteinuria (i.e. high level of protein loss in the urine), which indicates kidney disease.
Potential Application   Advantage
- The techniques are ready to develop into assay kits and portable devices. Research institutes (related to biology, biochemistry, and pharmacy) and clinical laboratories will be target users.

- The development of low-cost, portable, and disposable paper strips for urine detection will benefit health monitoring, especially in developing countries.
  1. Low cost (facile preparation procedures and cheap starting materials)
2. Sensitive nucleic acid stain with low toxicity and rapidity (Most of the commercial stains are mutagenic and take long time to finish the stain process)
3. Sensitive indicator for urinary protein detection (The detection limit of the products on market is still higher than the health threshold)
4. Sensor for high-throughput quadruplex-targeting anticancer drug screening (Fluorescent probes for quadruplex DNA are rare and in high demand owing to the accumulated evidence of its importance in cancer therapy)
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