Water-Soluble AIE Luminogen for Monitoring and Retardation of Amyloid Fibrillation of Insulin
Patent Information:
1. Filing Country: USA
Application No: 13/359514
Filing Date: 27 January 2012
Patent No: 8679738
Issue Date: 25 March 2014
2. Filing Country: China
Application No: 201210021921.7
Filing Date: 31 January 2012
Patent No: ZL201210021921.7
Issue Date: 12 August 2015
3. Filing Country: Hong Kong
Application No: 13101582.7
Filing Date: 05 February 2013
Patent No: HK1174687
Issue Date: 22 April 2016
4. Filing Country: USA
Application No: 14/171957
Filing Date: 04 February 2014
Patent No: 9279806
Issue Date: 08 March 2016
Inventor 1: Prof. Benzhong, TANG
Inventor 2: Dr. Yuning, HONG
Inventor 3: Ms. Sijie, CHEN

Based on the proposition that amyloid fibril formation is pathologically associated with many neurodegenerative diseases, this invention presents a fluorescent bioprobe for the monitoring of amyloid fibrillation process. The nonluminescent tetraphenylethylene derivative (BSPOTPE) is induced to emit intensely due to the restriction of intramolecular rotation, showing a novel phenomenon of aggregation-induced emission (AIE). The AIE-active BSPOTPE exhibits distinct fluorescent behaviours toward the native and fibrillar forms of insulin and thus enables the quantitative analysis and kinetic investigation of the fibrillation process. Another observation is that premixing BSPOTPE with insulin can extend the lag phase and decelerate the growth rate of insulin fibrillation, demonstrating the role of BSPOTPE as an effective retarder for amyloid fibrillation.
Potential Application   Advantage
The newly invented water-soluble AIE-active polyene compounds could be used for the detection and labeling of biomacromolecules. They are potential candidates for sensor device in biologic area.
As an indicator, the reagent can be used to monitor the fibrillation process of proteins.
As a retardant, the reagent can serve as a stabilizer for long-term storage of insulin for treatment of diabetes.
  1) Water soluble
2) Can act as both an indicator and a retardant
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Contact information :
Ms Shirley Woo
(852) 2358 7918
HKUST R and D Corporation Ltd
(852) 2358 7917
Technology Transfer Center
香港九龍清水灣 香港科技大學